AlphaBiolabs SARS-CoV-2 Whole Genome Sequencing Service
Sequencing is required for all Day 2 and Day 8 travel tests that are positive with a Ct value of less than 30. Sequencing results must be reported to DHSC within 120 hours of the original positive result report time.
Following sequencing, data will be assessed by our team of analysts to ensure data meets the quality standards required by DHSC. Upon completion, all relevant BAM files and data will be uploaded to the DHSC by AlphaBiolabs. Results will be sent via email to partners so the individuals’ sample details can be updated with PHE sequencing label (VOC/VUI) via SGSS or the PHE reporting system used.
All samples must be clearly labelled for sequencing, packaged as detailed below and sent to:
Unit 1 Webster Court
Customers should include a printed sample submission spreadsheet detailing all samples that will be received, in addition to emailing an electronic version to the laboratory at firstname.lastname@example.org upon dispatch.
Samples should only be sent for sequencing when testing positive on RT-PCR with ALL gene targets presenting at Ct below 30. There are two options for sending samples:
Clinical sample in VTM tube for re-extraction and sequencing:
- At least 500 µl of original VTM (can be in original tube)
- Barcode sticker must be vertical for scanning and sticker type must remain intact during cold transit. The barcode must be the same as that used to report the initial PCR test result so that sequencing and PCR results can be matched by PHE.
- Samples must be double-bagged (inner bag to contain absorbent material)
Extracted Sample Eluate (RNA) for sequencing:
- At least 30 µl of good quality, extracted viral RNA
- Samples must be contained in PCR clean, RNAse/DNAse free cryo-tubes with barcode sticker orientated vertically for scanning and sticker type must remain intact during cold transit. The barcode must be the same as that used to report the initial PCR test result so that sequencing and PCR results can be matched by PHE.
IMPORTANT: Any samples not prepared and shipped as detailed above may be refused by the laboratory for sequencing.
For all samples, please ensure the barcode matches the original sample ID from the initial RT-PCR test. The sample ID must be identical so PHE can merge the sequencing data with the original passenger test result and information.
Packages containing material for sequencing must be clearly labelled as samples for sequencing with your company name so they are not mistaken for PCR processing and we can add all barcodes onto our LIMS system under your company account for results reporting.
The laboratory is open and operational 24/7 to ensure a fast turn-around of sequencing results. For couriers, as the material is temperature sensitive, please ensure they ring the bell of Unit 1 so a member of the staff can receive the samples. The laboratory phone number can be provided, if required.